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KMID : 1059519930370110937
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Journal of the Korean Chemical Society
1993 Volume.37 No. 11 p.937 ~ p.942
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Amperometric Enzyme Electrode for the Determination of NH4+
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Seo Moo-Lyong
Kim Jae-Sang
Lee Shim-Sung
Bae Zun-Ung
Lee Heung-Lark
Park Tae-Myung
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Abstract
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Enzyme electrodes for amperometric measurement of ammonia was prepared by immobilization of L-glutamate dehydrogenase on an Immobilon-AV Affinity membrane and attachment to a glassy carbon electrode. Reduced nicotinamide adenine dinucleotide (NADH) was used as the electroactive species. The electrochemical oxidation of NADH was monitored at ＋1.0 volt vs. Ag/AgCl. Response was linear from 4.0 × 10-5 to 4.0 × 10-4 M. The detection limit was 2.0 × 10-6 M. Response time, the optimum pH and life time of enzyme immobilized membrane were 2 min, pH 7.3∼7.6 (Dulbecco's buffer solution) and about 25 days respectively. When the enzyme electrode was applied to the NH4+ determination with amperometric method, other physiological materials had no interference.
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